Publications

ZFTA-RELA ependymomas produce itaconate as an oncometabolite to sustain pathogenic fusion expression
Natarajan S, Skeans JH, Lum J, Nenwani M, Pun M, Sweha SR, Animasahun O, Narayanan P, Sunil S, Young E, Bayliss JM, Parolia A, Sajjakulnukit P, Eyunni S, Panwalkar P, Clausen M, Dang D, Hawes D, Yang F, Santi M, Judkins AR, Fudym Y, Vigil T, Franson A, Mortensen RM, Diamond M, Ozawa T, Griesinger A, Holland E, Foreman N, Michaelraj KA, Agnihotri S, Taylor M, Gilbertson RJ, Koschmann C, Chinnaiyan AM, Lyssiotis CA, Nagrath D, Venneti S.  Nature, accepted in principle.

Development and validation of digital twin approaches to quantify metabolic fluxes in human cancers
Meghdadi B, Al-Holou WN, Scott A, Mittal A, Liang N, Palavalasa S, Achreja A, Qi N, O’Brien A, Tarnal V, Zhou W, †Lyssiotis CA [†co-senior authors], †Wahl DR, †Nagrath D.  Cell Metabolism, in press.

Chemotherapy enhances HMGA1 secretion through the mutant p53-CK2 axis in pancreatic ductal adenocarcinoma cells
Danzi F, Butera G, Sutton D, Perricone MD, Hu Y, Celesia A, Manfredi M, Brandi J, Pourmandi N, Nelson N, Lin L, Bevere M, Pacchiana R, Pea A, Scarpa A, Luchini C, Cecconi D, Ugel S, Lyssiotis CA, Fiore A, Donadelli M.  Cell Death and Differentiation, accepted.

DUSP6 is upregulated in metastasis and influences migration and metabolism in pancreatic cancer cells
Ruckert MT, Walsh RM, Bye BB, Eades AE, Yan W, Bednar F, Shi J, Lyssiotis CA, VanSaun MN, Silveira VS.  Scientific Reports, 2025;15(1):33996.

IMPACT governs GCN1-mediated metastatic outgrowth of pancreatic cancer
Sinha S, Nwosu Z, Das RN, Ke X, Billel G, Van Beek E, Rossi A, Ayabe R, Wach M, McDonald JD, Ruff S, Verbus E, Saif A, Gupta S, Desai P, Ranjan A, Khan T, Pu T, Sarvestani L, Teke M, Remmert K, Smith E, Ryan C, Lambdin J, Luberice K, Gregory SN, Lux S, Hong H, Lin Y, Luna A, Alexander I, Akmal SR, Rehman S, Rainey A, Prickett T, Sindiri S, Koparde V, Sevilla S, King C, Sun Z, Bubunenko N, Li E, Cathleen H, Andresson T, Cam M, Wu X, Klemen ND, Blakely AM, Davis J, Trinchieri G, Singh P, Rosenberg SA, Pasca di Magliano M, Lyssiotis CA, Yaffe MB, Giancotti FG, Hernandez JM. Cancer Discovery, online ahead of print.

Gut sulfide metabolism modulates behavior and brain bioenergetics
Kumar R, Sykes DJ, Schaller ML, Patel R, Sajjakulnukit P, Singhal R, Wong H, Ichinose R, Lyssiotis CA, Shah YM, Banerjee R.  PNAS, 2025;122(25):e2503677122.

Rewiring of cortical glucose metabolism fuels human brain cancer growth
Scott AJ, Mittal A, Meghdadi B, Palavalasa S, Achreja A, O’Brien A, Kothari AU, Zhou W, Xu J, Lin A, Wilder-Romans K, Edwards DM, Wu Z, Feng J, Andren AC, Zhang L, Tarnal V, Qi N, Fischer J, Yang E, Regan MS, Stopka SA, Baquer G, Lawrence TS, Venneti S, Agar NYR, †Lyssiotis CA [†co-senior authors], †Al-Holou WN, †Nagrath D, †Daniel R. Wahl DR. Nature, 2025;646(8084):413-422.

» Highlighted in Nature News (2025) link
» Highlighted in Live Science (2025) link
» Highlighted in Nature News & Views (2025) 646:292-293.
» Highlighted in Cancer Discovery Research Watch (2025)

Isocitrate Dehydrogenase 1 Primes Group-3 Medulloblastomas For Cuproptosis 
Dang D, Deogharkar A, McKolay J, Smith K, Panwalkar P, Hoffman S, Ji S, Azambuja A, Natarajan SK, Lum J, Bayliss J, Manzeck K, Sweha SR, Hamanishi E, Pun M, Ji S, Patel D, Rua S, Animasahun O, Achreja A, Ogrodzinski MP, Cotter J, Hawes D, Yang F, Doherty R, Franson AT, Hanaford AR, Eberhart CG, Raabe EH, Orr BA, Wechsler-Reya RJ, Chen B, Lyssiotis CA, Shah YM, Lunt SY, Judkins AR, Prensner J, Koschmann C, Waszak S, Nagrath D, Simoes-Costa, M, Northcott P, Venneti S. Cancer Cell, 2025;43(6):1159-1174.e8.

Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer
Cheng C, Hu J, Mannan R, Bhattacharyya R, Rossiter NJ, Magnuson B, Wisniewski JP, Zheng Y, Xiao L, Li C, Awad D, Korkaya A, He T, Bao Y, Zhang Y, Cao X, Wang Z, Mehra R, Morlacchi P, Sahai V, Pasca di Magliano M, Ding K, †Qiao Y, †Lyssiotis CA [†co-senior authors], †Chinnaiyan AM.  Nature, 2025;642(8068):776-784.

A luminescent based protocol for NAD+/NADH detection in C. elegans, mice, and human whole blood samples
Wang H, Zhang J, Cao S, Lagartos-Donate MJ, Zhang S, Lautrup S, Hu Z, Lyssiotis CA, Houtkooper RH, Fang EF.  STAR Protocols, 2024;5(4):103428.

Multi-dimensional analyses identify genes of high priority for pancreatic cancer research
Nwosu ZC, Giza H, Nassif M, Charlestin V, Menjivar RE, Kim D, Kemp SB, Steele NG, Hu J, Hu B, Wang S, †Pasca di Magliano M, †Lyssiotis CA. [†co-senior authors]  JCI Insight, 2025;10(4):e174264.

Glutaminase deficiency in rod photoreceptors disrupts nonessential amino acid levels to activate the integrated stress response and induce rapid
Goswami MT, Weh E, Subramanya S, Weh KM, Durumutla HB, Hager H, Miller N, Chaudhury S, Andren A, Sajjakulnukit P, Besirli CG, Lyssiotis CA, Wubben TJ.  degeneration. eLife, 2025;10.7554/eLife.100747.2

BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the Coenzyme Q redox state
Chen B, Nguyen TM, Jadhav P, Halligan BS, Rossiter NJ, Guerra RM, Koo I, Hanna DA, Lin J, Banerjee R, Pagliarini DJ, Patterson AD, Mosalaganti S, Sexton JZ, Calì T, †Lyssiotis CA [†co-senior authors], †Shah YM
Molecular Cell, accepted

Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis
Chen B, Lyssiotis CA, Shah YM. Mol Cell. 2025 Apr 17;85(8):1487-1508. doi: 10.1016/j.molcel.2025.03.003. PMID: 40250411.

A genome-wide screen identifies genes required for erythroid differentiation
Myers G, Friedman A, Yu L, Pourmandi N, Kerpet C, Ito MA, Saba R, Tang V, Ozel AB, Bergin IL, Johnson CN, Ku CJ, Wang Y, Balbin-Cuesta G, Lim KC, Lin Z, Drysdale C, McGee B, Kurita R, Nakamura Y, Liu X, Siemieniak D, Singh SA, Lyssiotis CA, Maillard I, Weisman LS, Engel JD, Khoriaty R. Nat Commun. 2025 Apr 12;16(1):3488. doi: 10.1038/s41467-025-58739-w. PMID: 40221460; PMCID: PMC11993733.

Correction: Clinical Efficacy of ONC201 in H3K27M-Mutant Diffuse Midline Gliomas Is Driven by Disruption of Integrated Metabolic and Epigenetic Pathways
Venneti S, Kawakibi AR, Ji S, Waszak SM, Sweha SR, Mota M, Pun M, Deogharkar A, Chung C, Tarapore RS, Ramage S, Chi A, Wen PY, Arrillaga-Romany I, Batchelor TT, Butowski NA, Sumrall A, Shonka N, Harrison RA, de Groot J, Mehta M, Hall MD, Daghistani D, Cloughesy TF, Ellingson BM, Beccaria K, Varlet P, Kim MM, Umemura Y, Garton H, Franson A, Schwartz J, Jain R, Kachman M, Baum H, Burant CF, Mottl SL, Cartaxo RT, John V, Messinger D, Qin T, Peterson E, Sajjakulnukit P, Ravi K, Waugh A, Walling D, Ding Y, Xia Z, Schwendeman A, Hawes D, Yang F, Judkins AR, Wahl D, Lyssiotis CA, Nava D, Alonso MM, Eze A, Spitzer J, Schmidt SV, Duchatel RJ, Dun MD, Cain JE, Jiang L, Stopka SA, Baquer G, Regan MS, Filbin MG, Agar NYR, Zhao L, Kumar-Sinha C, Mody R, Chinnaiyan A, Kurokawa R, Pratt D, Yadav VN, Grill J, Kline C, Mueller S, Resnick A, Nazarian J, Allen JE, Odia Y, Gardner SL, Koschmann C. Cancer Discov. 2025 Mar 3;15(3):657. doi: 10.1158/2159-8290.CD-25-0208. Erratum for: Cancer Discov. 2023 Nov 1;13(11):2370-2393. doi: 10.1158/2159-8290.CD-23-0131. PMID: 40025951; PMCID: PMC11873718.

Glutamine catabolism supports amino acid biosynthesis and suppresses the integrated stress response to promote photoreceptor survival
Goswami MT, Weh E, Subramanya S, Weh KM, Durumutla HB, Hager H, Miller N, Chaudhury S, Andren A, Sajjakulnukit P, Zhang L, Besirli CG, Lyssiotis CA, Wubben TJ. bioRxiv [Preprint]. 2025 Feb 25:2024.03.26.582525. doi: 10.1101/2024.03.26.582525. PMID: 38586045; PMCID: PMC10996599.

Itaconate potentiates hepatic gluconeogenesis through NRF2 induction
El-Derany MO, Ramakrishnan SK, Li Y, Buscher K, Jarad CA, Schaller ML, Cantwell M, Vigil TM, Frieler RA, Sajjakulnukit P, Lyssiotis CA, Mortensen RM, Shah YM. PLoS One. 2025 May 5;20(5):e0322946. doi: 10.1371/journal.pone.0322946. PMID: 40323920; PMCID: PMC12052187.

Subcellular mitochondrial heterogeneity enables opposing metabolic demands
Chen B, Shah YM, Lyssiotis CA. Trends Endocrinol Metab. 2025 Mar;36(3):202-204. doi: 10.1016/j.tem.2025.01.003. Epub 2025 Jan 28. PMID: 39880788; PMCID: PMC11879766.

Lipidomics profiling identifies β-oxidation as a key process in noise-induced hearing loss
Wallace G, Ji L, Cassinotti LR, Kachman M, Lyssiotis CA, Burant CF, Corfas G. bioRxiv [Preprint]. 2025 Mar 26:2025.03.25.645361. doi: 10.1101/2025.03.25.645361. PMID: 40196644; PMCID: PMC11974867.

Multidimensional analyses identify genes of high priority for pancreatic cancer research
Nwosu ZC, Giza HM, Nassif M, Charlestin V, Menjivar RE, Kim D, Kemp SB, Sajjakulnukit P, Andren A, Zhang L, Lai WK, Loveless I, Steele N, Hu J, Hu B, Wang S, Pasca di Magliano M, Lyssiotis CA. JCI Insight. 2025 Jan 7;10(4):e174264. doi: 10.1172/jci.insight.174264. PMID: 39774001; PMCID: PMC11949049.

Isocitrate dehydrogenase 1 primes group-3 medulloblastomas for cuproptosis
Dang D, Deogharkar A, McKolay J, Smith KS, Panwalkar P, Hoffman S, Tian W, Ji S, Azambuja AP, Natarajan SK, Lum J, Bayliss J, Manzeck K, Sweha SR, Hamanishi E, Pun M, Patel D, Rau S, Animasahun O, Achreja A, Ogrodzinski MP, Diessl J, Cotter J, Hawes D, Yang F, Doherty R, Franson AT, Hanaford AR, Eberhart CG, Raabe EH, Orr BA, Wechsler-Reya RJ, Chen B, Lyssiotis CA, Shah YM, Lunt SY, Banerjee R, Judkins AR, Prensner JR, Koschmann C, Waszak SM, Nagrath D, Simoes-Costa M, Northcott PA, Venneti S. Cancer Cell. 2025 May 12:S1535-6108(25)00172-2. doi: 10.1016/j.ccell.2025.04.013. Epub ahead of print. PMID: 40378837.

The Hallmarks of Precancer
Stangis MM, Chen Z, Min J, Glass SE, Jackson JO, Radyk MD, Hoi XP, Brennen WN, Yu M, Dinh HQ, Coffey RJ, Shrubsole MJ, Chan KS, Grady WM, Yegnasubramanian S, Lyssiotis CA, Maitra A, Halberg RB, Dey N, Lau KS. Cancer Discov. 2024 Apr 4;14(4):683-689. doi: 10.1158/2159-8290.CD-23-1550. PMID: 38571435; PMCID: PMC11170686.

Subset-specific mitochondrial stress and DNA damage shape T cell responses to fever and inflammation
Heintzman DR, Sinard RC, Fisher EL, Ye X, Patterson AR, Elasy JH, Voss K, Chi C, Sugiura A, Rodriguez-Garcia GJ, Chowdhury NU, Arner EN, Krystoviak ES, Mason FM, Toudji YT, Steiner KK, Khan W, Olson LM, Jones AL, Hong HS, Bass L, Beier KL, Deng W, Lyssiotis CA, Newcomb DC, Bick AG, Rathmell WK, Wilson JT, Rathmell JC. Sci Immunol. 2024 Sep 20;9(99):eadp3475. doi: 10.1126/sciimmunol.adp3475. Epub 2024 Sep 20. PMID: 39303018; PMCID: PMC11607909.

A call for accessible tools to unlock single-cell immunometabolism research
Cosgrove J, Marçais A, Hartmann FJ, Bergthaler A, Zanoni I, Corrado M, Perié L, Cabezas-Wallscheid N, Bousso P, Alexandrov T, Kielian T, Martínez-Martín N, Opitz CA, Lyssiotis CA, Argüello RJ, Van den Bossche J. Nat Metab. 2024 May;6(5):779-782. doi: 10.1038/s42255-024-01031-w. PMID: 38605184.

GTP Signaling Links Metabolism, DNA Repair, and Responses to Genotoxic Stress
Zhou W, Zhao Z, Lin A, Yang JZ, Xu J, Wilder-Romans K, Yang A, Li J, Solanki S, Speth JM, Walker N, Scott AJ, Wang L, Wen B, Andren A, Zhang L, Kothari AU, Yao Y, Peterson ER, Korimerla N, Werner CK, Ullrich A, Liang J, Jacobson J, Palavalasa S, O'Brien AM, Elaimy AL, Ferris SP, Zhao SG, Sarkaria JN, Győrffy B, Zhang S, Al-Holou WN, Umemura Y, Morgan MA, Lawrence TS, Lyssiotis CA, Peters-Golden M, Shah YM, Wahl DR. Cancer Discov. 2024 Jan 12;14(1):158-175. doi: 10.1158/2159-8290.CD-23-0437. PMID: 37902550; PMCID: PMC10872631.

A metabolic atlas of mouse aging
Pilley SE, Awad D, Latumalea D, Esparza E, Zhang L, Shi X, Unfried M, Wang S, Mulondo R, Kashyap SB, Moaddeli D, Sajjakulnukit P, Sutton D, Wong H, Coakley AJ, Garcia G, Higuchi-Sanabria R, Liu S, Yu B, Tu WB, Kennedy BK, Lyssiotis CA, Mullen PJ. bioRxiv [Preprint]. 2024 May 5:2024.05.04.592445. doi: 10.1101/2024.05.04.592445. PMID: 38746230; PMCID: PMC11092783.

Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection
Reynolds MB, Klein B, McFadden MJ, Judge NK, Navarrete HE, Michmerhuizen BC, Awad D, Schultz TL, Harms PW, Zhang L, O'Meara TR, Sexton JZ, Lyssiotis CA, Kahlenberg JM, O'Riordan MX. Cell Rep. 2024 Aug 27;43(8):114607. doi: 10.1016/j.celrep.2024.114607. Epub 2024 Aug 9. PMID: 39126652; PMCID: PMC11590196.

Sulfide oxidation promotes hypoxic angiogenesis and neovascularization
Kumar R, Vitvitsky V, Sethaudom A, Singhal R, Solanki S, Alibeckoff S, Hiraki HL, Bell HN, Andren A, Baker BM, Lyssiotis CA, Shah YM, Banerjee R. Nat Chem Biol. 2024 Oct;20(10):1294-1304. doi: 10.1038/s41589-024-01583-8. Epub 2024 Mar 20. PMID: 38509349; PMCID: PMC11584973.

Opposing diet, microbiome, and metabolite mechanisms regulate inflammatory bowel disease in a genetically susceptible host
Pereira GV, Boudaud M, Wolter M, Alexander C, De Sciscio A, Grant ET, Trindade BC, Pudlo NA, Singh S, Campbell A, Shan M, Zhang L, Yang Q, Willieme S, Kim K, Denike-Duval T, Fuentes J, Bleich A, Schmidt TM, Kennedy L, Lyssiotis CA, Chen GY, Eaton KA, Desai MS, Martens EC. Cell Host Microbe. 2024 Apr 10;32(4):527-542.e9. doi: 10.1016/j.chom.2024.03.001. Epub 2024 Mar 20. PMID: 38513656; PMCID: PMC11064055.

Metabolic landscape of the healthy pancreas and pancreatic tumor microenvironment
Bonilla ME, Radyk MD, Perricone MD, Elhossiny AM, Harold AC, Medina-Cabrera PI, Kadiyala P, Shi J, Frankel TL, Carpenter ES, Green MD, Mitrea C, Lyssiotis CA, Pasca di Magliano M. JCI Insight. 2024 Aug 13;9(18):e180114. doi: 10.1172/jci.insight.180114. PMID: 39315547; PMCID: PMC11457849.

Iron regulates the quiescence of naive CD4 T cells by controlling mitochondria and cellular metabolism
Kumar A, Ye C, Nkansah A, Decoville T, Fogo GM, Sajjakulnukit P, Reynolds MB, Zhang L, Quaye O, Seo YA, Sanderson TH, Lyssiotis CA, Chang CH. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2318420121. doi: 10.1073/pnas.2318420121. Epub 2024 Apr 15. PMID: 38621136; PMCID: PMC11047099.

Development of an orally bioavailable mSWI/SNF ATPase degrader and acquired mechanisms of resistance in prostate cancer
He T, Cheng C, Qiao Y, Cho H, Young E, Mannan R, Mahapatra S, Miner SJ, Zheng Y, Kim N, Zeng VZ, Wisniewski JP, Hou S, Jackson B, Cao X, Su F, Wang R, Chang Y, Kuila B, Mukherjee S, Dukare S, Aithal KB, D S S, Abbineni C, Vaishampayan U, Lyssiotis CA, Parolia A, Xiao L, Chinnaiyan AM. Proc Natl Acad Sci U S A. 2024 Apr 9;121(15):e2322563121. doi: 10.1073/pnas.2322563121. Epub 2024 Apr 1. PMID: 38557192; PMCID: PMC11009648.

Therapeutic targeting of differentiation-state dependent metabolic vulnerabilities in diffuse midline glioma
Mbah NE, Myers AL, Sajjakulnukit P, Chung C, Thompson JK, Hong HS, Giza H, Dang D, Nwosu ZC, Shan M, Sweha SR, Maydan DD, Chen B, Zhang L, Magnuson B, Zhu Z, Radyk M, Lavoie B, Yadav VN, Koo I, Patterson AD, Wahl DR, Franchi L, Agnihotri S, Koschmann CJ, Venneti S, Lyssiotis CA. Nat Commun. 2024 Oct 17;15(1):8983. doi: 10.1038/s41467-024-52973-4. PMID: 39419964; PMCID: PMC11487135.
 

γ-aminobutyric acid receptor B signaling drives glioblastoma in females in an immune-dependent manner
Pathak A, Palasalava S, Knott MV, Colon B, Ciervo E, Zhou Y, Mitchell J, Pumar OT, Wong HKA, Zhang L, Susic N, Shah KH, Kay K, Chin D, Johnson S, Cheng F, Lyssiotis CA, Watson DC, Ceccarelli M, Shah A, Wahl DR, Lathia JD, Bayik D. bioRxiv[Preprint]. 2024 Jul 22:2024.07.18.603996. doi: 10.1101/2024.07.18.603996. PMID: 39091833; PMCID: PMC11291093.

Itaconate suppresses house dust mite-induced allergic airways disease and Th2 cell differentiation
Li Y, Singh S, Breckenridge HA, Cui TX, Vigil TM, Kreger JE, Lei J, Wong HKA, Sajjakulnukit P, Zhou X, Kelley Bentley J, Lyssiotis CA, Mortensen RM, Hershenson MB. Mucosal Immunol. 2024 Dec;17(6):1174-1183. doi: 10.1016/j.mucimm.2024.08.001. Epub 2024 Aug 13. PMID: 39147278; PMCID: PMC11967901.

Neutrophil glucose flux as a therapeutic target in antiphospholipid syndrome
Tambralli A, Harbaugh A, NaveenKumar SK, Radyk MD, Rysenga CE, Sabb K, Hurley JM, Sule GJ, Yalavarthi S, Estes SK, Hoy CK, Smith T, Sarosh C, Madison JA, Schaefer JK, Sood SL, Zuo Y, Sawalha AH, Lyssiotis CA, Knight JS. J Clin Invest. 2024 Jun 13;134(15):e169893. doi: 10.1172/JCI169893. PMID: 38869951; PMCID: PMC11290966.

Purine salvage promotes treatment resistance in H3K27M-mutant diffuse midline glioma
Peterson ER, Sajjakulnukit P, Scott AJ, Heaslip C, Andren A, Wilder-Romans K, Zhou W, Palavalasa S, Korimerla N, Lin A, O'Brien A, Kothari A, Zhao Z, Zhang L, Morgan MA, Venneti S, Koschmann C, Jabado N, Lyssiotis CA, Castro MG, Wahl DR. Cancer Metab. 2024 Apr 9;12(1):11. doi: 10.1186/s40170-024-00341-7. PMID: 38594734; PMCID: PMC11003124.

Quiescence enables unrestricted cell fate in naive embryonic stem cells
Khoa LTP, Yang W, Shan M, Zhang L, Mao F, Zhou B, Li Q, Malcore R, Harris C, Zhao L, Rao RC, Iwase S, Kalantry S, Bielas SL, Lyssiotis CA, Dou Y. Nat Commun. 2024 Feb 26;15(1):1721. doi: 10.1038/s41467-024-46121-1. Erratum in: Nat Commun. 2024 Mar 12;15(1):2215. doi: 10.1038/s41467-024-46566-4. PMID: 38409226; PMCID: PMC10897426.

ER-associated degradation adapter Sel1L is required for CD8⁺ T cell function and memory formation following acute viral infection
Correa-Medero LO, Jankowski SE, Hong HS, Armas ND, Vijendra AI, Reynolds MB, Fogo GM, Awad D, Dils AT, Inoki KA, Williams RG, Ye AM, Svezhova N, Gomez-Rivera F, Collins KL, O'Riordan MX, Sanderson TH, Lyssiotis CA, Carty SA. Cell Rep. 2024 May 28;43(5):114156. doi: 10.1016/j.celrep.2024.114156. Epub 2024 Apr 29. PMID: 38687642; PMCID: PMC11194752.

Fibrotic tumors tune metabolism for immune evasion
Perricone MD, Lyssiotis CA. Nat Cancer. 2024 Jul;5(7):955-957. doi: 10.1038/s43018-024-00758-5. PMID: 38831057.

Purine Metabolism is a Metabolic Vulnerability of Cancers with Low p16 Expression
Tangudu NK, Buj R, Wang H, Wang J, Cole AR, Uboveja A, Fang R, Amalric A, Yang B, Chatoff A, Crispim CV, Sajjakulnukit P, Lyons MA, Cooper K, Hempel N, Lyssiotis CA, Chandran UR, Snyder NW, Aird KM. De Novo. Cancer Res Commun. 2024 May 2;4(5):1174-1188. doi: 10.1158/2767-9764.CRC-23-0450. PMID: 38626341; PMCID: PMC11064835.

Targeting PIKfyve-driven lipid homeostasis as a metabolic vulnerability in pancreatic cancer

Cheng C, Hu J, Mannan R, Bhattacharyya R, Rossiter NJ, Magnuson B, Wisniewski JP, Zheng Y, Xiao L, Li C, Awad D, He T, Bao Y, Zhang Y, Cao X, Wang Z, Mehra R, Morlacchi P, Sahai V, di Magliano MP, Shah YM, Ding K, Qiao Y, Lyssiotis CA, Chinnaiyan AM. bioRxiv [Preprint]. 2024 Mar 20:2024.03.18.585580. doi: 10.1101/2024.03.18.585580. PMID: 38562800; PMCID: PMC10983929.

Engineering immunity using metabolically active polymeric nanoparticles
Griffin KV, Saunders MN, Lyssiotis CA, Shea LD. Trends Biotechnol. 2024 Dec 27:S0167-7799(24)00345-7. doi: 10.1016/j.tibtech.2024.11.016. Epub ahead of print. PMID: 39732608.

A luminescent-based protocol for NAD⁺/NADH detection in C. elegans, mice, and human whole blood
Wang HL, Zhang J, Cao SQ, Lagartos-Donate MJ, Zhang SQ, Lautrup S, Hu Z, Lyssiotis CA, Houtkooper RH, Fang EF. STAR Protoc. 2024 Dec 20;5(4):103428. doi: 10.1016/j.xpro.2024.103428. Epub 2024 Nov 1. PMID: 39487980; PMCID: PMC11567065.

Ras-dependent activation of BMAL2 regulates hypoxic metabolism in pancreatic cancer
Maurer HC, Garcia-Curiel A, Holmstrom SR, Castillo C, Palermo CF, Sastra SA, Andren A, Zhang L, Le Large TYS, Sagalovskiy I, Ross DR, Wong W, Shaw K, Genkinger J, Manji GA, Iuga AC, Schmid RM, Johnson K, Badgley MA, Lyssiotis CA, Shah Y, Califano A, Olive KP. bioRxiv[Preprint]. 2024 Jun 22:2023.03.19.533333. doi: 10.1101/2023.03.19.533333. PMID: 36993718; PMCID: PMC10055246.

BRD4-mediated epigenetic regulation of endoplasmic reticulum-mitochondria contact sites is governed by the mitochondrial complex III
Chen B, Stark DC, Jadhav PV, Lynn-Nguyen TM, Halligan BS, Rossiter NJ, Sindoni N, Shin M, Paulo JA, Chang M, Koo I, Koshkin S, Eyunni S, Ronchi P, Paulsen MT, Morlacchi P, Hanna DA, Lin J, Guerra RM, Pagliarini DJ, Banerjee R, Parolia A, Ljungman ME, Patterson AD, Mancias JD, Mosalaganti S, Sexton JZ, Calì T, Lyssiotis CA, Shah YM. bioRxiv [Preprint]. 2024 Nov 5:2024.02.02.578646. doi: 10.1101/2024.02.02.578646. PMID: 38352460; PMCID: PMC10862858.

Reciprocal links between methionine metabolism, DNA repair and therapy resistance in glioblastoma
Korimerla N, Meghdadi B, Haq I, Wilder-Romans K, Xu J, Becker N, Zhu Z, Kalev P, Qi N, Evans C, Kachman M, Zhao Z, Lin A, Scott AJ, O'Brien A, Kothari A, Sajjakulnukit P, Zhang L, Palavalasa S, Peterson ER, Hyer ML, Marjon K, Sleger T, Morgan MA, Lyssiotis CA, Stone EM, Ferris SP, Lawrence TS, Nagrath D, Zhou W, Wahl DR. bioRxiv [Preprint]. 2024 Nov 21:2024.11.20.624542. doi: 10.1101/2024.11.20.624542. PMID: 39651281; PMCID: PMC11623687.

Epigenetic Reprogramming of Autophagy Drives Mutant IDH1 Glioma Progression and Response to Radiation
Núñez FJ, Banerjee K, Mujeeb AA, Mauser A, Tronrud CE, Zhu Z, Taher A, Kadiyala P, Carney SV, Garcia-Fabiani MB, Comba A, Alghamri MS, McClellan BL, Faisal SM, Nwosu ZC, Hong HS, Qin T, Sartor MA, Ljungman M, Cheng SY, Appelman HD, Lowenstein PR, Lahann J, Lyssiotis CA, Castro MG. bioRxiv [Preprint]. 2024 Mar 13:2024.03.08.584091. doi: 10.1101/2024.03.08.584091. PMID: 38559270; PMCID: PMC10979892.

Development of an orally bioavailable mSWI/SNF ATPase degrader and acquired mechanisms of resistance in prostate cancer
He T, Cheng C, Qiao Y, Cho H, Young E, Mannan R, Mahapatra S, Miner SJ, Zheng Y, Kim N, Zeng VZ, Wisniewski JP, Hou S, Jackson B, Cao X, Su F, Wang R, Chang Y, Kuila B, Mukherjee S, Dukare S, Aithal KB, D S S, Abbineni C, Lyssiotis CA, Parolia A, Xiao L, Chinnaiyan AM. bioRxiv [Preprint]. 2024 Mar 2:2024.02.29.582768. doi: 10.1101/2024.02.29.582768. Update in: Proc Natl Acad Sci U S A. 2024 Apr 9;121(15):e2322563121. doi: 10.1073/pnas.2322563121. PMID: 38464081; PMCID: PMC10925251.

Malic enzyme 1 knockout has no deleterious phenotype and is favored in the male germline under standard laboratory conditions
Alektiar JM, Shan M, Radyk MD, Zhang L, Halbrook CJ, Lin L, Espinoza C, Mier IF, Lavoie BL, Salvatore L, Pasca di Magliano M, Cantley LC, Mueller JL, Lyssiotis CA. PLoS One. 2024 Jun 6;19(6):e0303577. doi: 10.1371/journal.pone.0303577. PMID: 38843233; PMCID: PMC11156412.

Quiescence enables unrestricted cell fate in naive embryonic stem cells
Khoa LTP, Yang W, Shan M, Zhang L, Mao F, Zhou B, Li Q, Malcore R, Harris C, Zhao L, Rao RC, Iwase S, Kalantry S, Bielas SL, Lyssiotis CA, Dou Y. Author Correction:. Nat Commun. 2024 Mar 12;15(1):2215. doi: 10.1038/s41467-024-46566-4. Erratum for: Nat Commun. 2024 Feb 26;15(1):1721. doi: 10.1038/s41467-024-46121-1. PMID: 38472240; PMCID: PMC10933312.

Isolation and characterization of microbiota from human pancreatic tumors and small intestine
Awad D, Attebury H, Hong R, Kim K, Zhang L, Bischoff A, deDekker A, Hoostal M, Nieto Carrion JA, Nelson NS, Strayhorn C, Frankel T, di Magliano MP, Lyssiotis CA, Schmidt TM, Daley D. bioRxiv [Preprint]. 2024 Dec 3:2024.11.27.625762. doi: 10.1101/2024.11.27.625762. PMID: 39677718; PMCID: PMC11642792.

Glucose-6-phosphate dehydrogenase deficiency accelerates pancreatic acinar-to-ductal metaplasia
Radyk MD, Nelson BS, Halbrook CJ, Wood A, Lavoie B, Salvatore L, Corfas G, Colacino JA, Shah YM, Crawford HC, Lyssiotis CA. bioRxiv
Abstract | PDF

Pancreatic cancer: Advances and challenges
Halbrook CJ, Lyssiotis CA, Pasca di Magliano M, Maitra A. Cell (2023) 186(8):1729-1754.
Abstract | PDF

Spotlight on GOT2 in Cancer Metabolism
Kerk SA, Garcia-Bermudez J, Birsoy K, Sherman MH, Shah YM, Lyssiotis CA. Onco Targets Ther. (2023) Aug 22;16:695-702.
doi: 10.2147/OTT.S382161. PMID: 37635751; PMCID: PMC10460182.
Abstract | PDF

Uridine-derived ribose fuels glucose-restricted pancreatic cancer
Nwosu ZC, Ward MH, Sajjakulnukit P, Poudel P, Ragulan C, Kasperek S, Radyk M, Sutton D, Menjivar RE, Andren A, Apiz-Saab JJ, Tolstyka Z, Brown K, Lee HJ, Dzierozynski LN, He X, Ps H, Ugras J, Nyamundanda G, Zhang L, Halbrook CJ, Carpenter ES, Shi J, Shriver LP, Patti GJ, Muir A, Pasca di Magliano M, Sadanandam A, Lyssiotis CA. Nature (2023) Jun;618(7963):151-158.
Abstract | PDF

Metabolomic Profiles of Human Glioma Inform Patient Survival
Scott AJ, Correa LO, Edwards DM, Sun Y, Ravikumar V, Andren AC, Zhang L, Srinivasan S, Jairath N, Verbal K, Muraszko K, Sagher O, Carty SA, Hervey-Jumper S, Orringer D, Kim MM, Junck L, Umemura Y, Leung D, Venneti S, Camelo-Piragua S, Lawrence TS, Ippolito JE, Al-Holou WN, Chinnaiyan P, Heth J, Rao A, Lyssiotis CA, Wahl DR. Antioxid Redox Signal. (2023) Nov;39(13-15):942-956.
doi: 10.1089/ars.2022.0085. Epub 2023 Apr 27. Erratum in: Antioxid Redox Signal. 2024 Apr 18;: PMID: 36852494; PMCID: PMC10655010.
Abstract | PDF

PTEN-induced kinase PINK1 supports colorectal cancer growth by regulating the labile iron pool
Chen B, Das NK, Talukder I, Singhal R, Castillo C, Andren A, Mancias JD, Lyssiotis CA, Shah YM. J Biol Chem. (2023) May;299(5):104691.
doi: 10.1016/j.jbc.2023.104691. Epub 2023 Apr 8. PMID: 37037306; PMCID: PMC10196865.
Abstract | PDF

Nutrient transporters: connecting cancer metabolism to therapeutic opportunities
Nwosu ZC, Song MG, di Magliano MP, Lyssiotis CA, Kim SE. Oncogene (2023) Mar;42(10):711-724.
doi: 10.1038/s41388-023-02593-x. Epub 2023 Feb 4. PMID: 36739364; PMCID: PMC10266237.
Abstract | PDF

OXPHOS promotes apoptotic resistance and cellular persistence in TH17 cells in the periphery and tumor microenvironment
Hong HS, Mbah NE, Shan M, Loesel K, Lin L, Sajjakulnukit P, Correa LO, Andren A, Lin J, Hayashi A, Magnuson B, Chen J, Li Z, Xie Y, Zhang L, Goldstein DR, Carty SA, Lei YL, Opipari AW, Argüello RJ, Kryczek I, Kamada N, Zou W, Franchi L, Lyssiotis CA. Sci Immunol. (2022) Nov 25;7(77):eabm8182.
Abstract | PDF

Differential integrated stress response and asparagine production drive symbiosis and therapy resistance of pancreatic adenocarcinoma cells
Halbrook CJ, Thurston G, Boyer S, Anaraki C, Jiménez JA, McCarthy A, Steele NG, Kerk SA, Hong HS, Lin L, Law FV, Felton C, Scipioni L, Sajjakulnukit P, Andren A, Beutel AK, Singh R, Nelson BS, Van Den Bergh F, Krall AS, Mullen PJ, Zhang L, Batra S, Morton JP, Stanger BZ, Christofk HR, Digman MA, Beard DA, Viale A, Zhang J, Crawford HC, Pasca di Magliano M, Jorgensen C, Lyssiotis CA. Nat Cancer (2022) Nov;3(11):1386-1403.
Abstract | PDF

Amino acid metabolism in primary bone sarcomas
Jiménez JA, Lawlor ER, Lyssiotis CA. Front Oncol. (2022) Oct 5;12:1001318.
doi: 10.3389/fonc.2022.1001318. PMID: 36276057; PMCID: PMC9581121.
Abstract | PDF

Metabolic requirement for GOT2 in pancreatic cancer depends on environmental context
Kerk SA, Lin L, Myers AL, Sutton DJ, Andren A, Sajjakulnukit P, Zhang L, Zhang Y, Jiménez JA, Nelson BS, Chen B, Robinson A, Thurston G, Kemp SB, Steele NG, Hoffman MT, Wen HJ, Long D, Ackenhusen SE, Ramos J, Gao X, Nwosu ZC, Galban S, Halbrook CJ, Lombard DB, Piwnica-Worms DR, Ying H, Pasca di Magliano M, Crawford HC, Shah YM, Lyssiotis CA. Elife (2022) Jul 11;11:e73245.
Abstract | PDF

Targeting allosteric regulation of cancer metabolism
Kremer DM, Lyssiotis CA. Nat Chem Biol. (2022) May;18(5):441-450. doi: 10.1038/s41589-022-00997-6. Epub 2022 Apr 28. PMID: 35484254.
Abstract | PDF

Discovery and Characterization of a Novel Allosteric Small-Molecule Inhibitor of NADP+-Dependent Malic Enzyme 1
Yoshida T, Kawabe T, Cantley LC, Lyssiotis CA. Biochemistry (2022) Aug 2;61(15):1548-1553.
doi: 10.1021/acs.biochem.2c00123. Epub 2022 Jul 12. PMID: 35819845; PMCID: PMC9352307.
Abstract | PDF

Multiomic characterization of pancreatic cancer-associated macrophage polarization reveals deregulated metabolic programs driven by the GM-CSF-PI3K pathway. Boyer S, Lee HJ, Steele N, Zhang L, Sajjakulnukit P, Andren A, Ward MH, Singh R, Basrur V, Zhang Y, Nesvizhskii AI, Pasca di Magliano M, Halbrook CJ, Lyssiotis CA. Elife. 2022 Feb 14;11:e73796. doi: 10.7554/eLife.73796. PMID: 35156921
Abstract

In an era of ctDNA, is metabolomics the new kid on the block? 
Van Tine BA, Lyssiotis CA. Cancer Res. 2022 Feb 9:clincanres.4180.2022. doi: 10.1158/1078-0432.CCR-21-4180. Epub ahead of print. PMID: 35140120.
Abstract

Metabolic regulation of ferroptosis in the tumor microenvironment
Mbah NE, Lyssiotis CA. J Biol Chem. 2022 Jan 20:101617. doi: 10.1016/j.jbc.2022.101617. Epub ahead of print. PMID: 35065965.
Abstract

Reuterin in the healthy gut microbiome suppresses colorectal cancer growth through altering redox balance
Bell HN, Rebernick RJ, Goyert J, Singhal R, Kuljanin M, Kerk SA, Huang W, Das NK, Andren A, Solanki S, Miller SL, Todd PK, Fearon ER, Lyssiotis CA, Gygi SP, Mancias JD, Shah YM. Cancer Cell. 2022 Feb 14;40(2):185-200.e6. doi: 10.1016/j.ccell.2021.12.001. Epub 2021 Dec 23. PMID: 34951957; PMCID: PMC8847337.
Abstract

A redox cycle with complex II prioritizes sulfide quinone oxidoreductase-dependent H2S oxidation
Kumar R, Landry AP, Guha A, Vitvitsky V, Lee HJ, Seike K, Reddy P, Lyssiotis CA, Banerjee R. J Biol Chem. 2022 Jan;298(1):101435. doi: 10.1016/j.jbc.2021.101435. Epub 2021 Nov 19. PMID: 34808207
Abstract

Multi-dimensional analyses identify genes of high priority for pancreatic cancer research
Nwosu ZC, Giza H, Nassif M, Charlestin V, Menjivar RE, Kim D, Kemp SB, Steele NG, Hu J, Hu B, Wang S, †Pasca di Magliano M, †Lyssiotis CA. bioRxiv
Abstract | PDF

A guide to interrogating immunometabolism
Voss K, Hong HS, Bader JE, Sugiura A, Lyssiotis CA, Rathmell JC. Nat Rev Immunol. (2021) Oct;21(10):637-652.
Abstract | PDF

The biological underpinnings of therapeutic resistance in pancreatic cancer
Beatty GL, Werba G, Lyssiotis CA, Simeone DM.  Genes Dev. 2021 Jul 1;35(13-14):940-962. doi: 10.1101/gad.348523.121. Epub 2021 Jun 11. PMID: 34117095; PMCID: PMC8247606.
Abstract

Colorectal cancer cells utilize autophagy to maintain mitochondrial metabolism for cell proliferation under nutrient stress
Devenport SN, Singhal R, Radyk MD, Taranto JG, Kerk SA, Chen B, Goyert JW, Jain C, Das NK, Oravecz-Wilson K, Zhang L, Greenson JK, Chen YE, Soleimanpour SA, Reddy P, Lyssiotis CA, Shah YM.  JCI Insight. 2021 Jun 17;6(14):e138835. doi: 10.1172/jci.insight.138835. PMID: 34138755; PMCID: PMC8328084.
Abstract

Hepcidin sequesters iron to sustain nucleotide metabolism and mitochondrial function in colorectal cancer epithelial cells
Schwartz AJ, Goyert JW, Solanki S, Kerk SA, Chen B, Castillo C, Hsu PP, Do BT, Singhal R, Dame MK, Lee HJ, Spence JR, Lakhal-Littleton S, Vander Heiden MG, Lyssiotis CA, Xue X, Shah YM.  Nat Metab. 2021 Jul;3(7):969-982. doi: 10.1038/s42255-021-00406-7. Epub 2021 Jun 21. PMID: 34155415; PMCID: PMC8316354.
Abstract

Fatty acid synthesis is required for breast cancer brain metastasis
Ferraro GB, Ali A, Luengo A, Kodack DP, Deik A, Abbott KL, Bezwada D, Blanc L, Prideaux B, Jin X, Possada JM, Chen J, Chin CR, Amoozgar Z, Ferreira R, Chen I, Naxerova K, Ng C, Westermark AM, Duquette M, Roberge S, Lindeman NI, Lyssiotis CA, Nielsen J, Housman DE, Duda DG, Brachtel E, Golub TR, Cantley LC, Asara JM, Davidson SM, Fukumura D, Dartois VA, Clish CB, Jain RK, Vander Heiden MG.  Nat Cancer. 2021 Apr;2(4):414-428. doi: 10.1038/s43018-021-00183-y. Epub 2021 Apr 1. Erratum in: Nat Cancer. 2021 Nov;2(11):1243. PMID: 34179825; PMCID: PMC8223728.
Abstract

Metabolic networks in mutant KRAS-driven tumours: tissue specificities and the microenvironment
Kerk SA, Papagiannakopoulos T, Shah YM, Lyssiotis CA. Nat Rev Cancer. 2021 Aug;21(8):510-525. doi: 10.1038/s41568-021-00375-9. Epub 2021 Jul 9. PMID: 34244683.
Abstract

GOT1 inhibition promotes pancreatic cancer cell death by ferroptosis
Kremer DM, Nelson BS, Lin L, Yarosz EL, Halbrook CJ, Kerk SA, Sajjakulnukit P, Myers A, Thurston G, Hou SW, Carpenter ES, Andren AC, Nwosu ZC, Cusmano N, Wisner S, Mbah NE, Shan M, Das NK, Magnuson B, Little AC, Savani MR, Ramos J, Gao T, Sastra SA, Palermo CF, Badgley MA, Zhang L, Asara JM, McBrayer SK, di Magliano MP, Crawford HC, Shah YM, Olive KP, Lyssiotis CA.  Nat Commun. 2021 Aug 11;12(1):4860. doi: 10.1038/s41467-021-24859-2. PMID: 34381026; PMCID: PMC8357841.
Abstract

The human type 2 diabetes-specific visceral adipose tissue proteome and transcriptome in obesity 
Carruthers NJ, Strieder-Barboza C, Caruso JA, Flesher CG, Baker NA, Kerk SA, Ky A, Ehlers AP, Varban OA, Lyssiotis CA, Lumeng CN, Stemmer PM, O’Rourke RW. Sci Rep. 2021 Aug 30;11(1):17394. doi: 10.1038/s41598-021-96995-0. PMID: 34462518; PMCID: PMC8405693.
Abstract

Clinical Targeting of Altered Metabolism in High-Grade Glioma
Scott AJ, Lyssiotis CA, Wahl DR. Cancer J. 2021 Sep-Oct 01;27(5):386-394. doi: 10.1097/PPO.0000000000000550. PMID: 34570453; PMCID: PMC8480512.
Abstract

Cyst fluid metabolites distinguish malignant from benign pancreatic cysts
Shi J, Yi Z, Jin L, Zhao L, Raskind A, Yeomans L, Nwosu ZC, Simeone DM, Lyssiotis CA, Stringer KA, Kwon RS.  Neoplasia. 2021 Nov;23(11):1078-1088. doi: 10.1016/j.neo.2021.09.004. Epub 2021 Sep 25. PMID: 34583246; PMCID: PMC8479274.
Abstract

Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology
Fujiwara H, Seike K, Brooks MD, Mathew AV, Kovalenko I, Pal A, Lee HJ, Peltier D, Kim S, Liu C, Oravecz-Wilson K, Li L, Sun Y, Byun J, Maeda Y, Wicha MS, Saunders TL, Rehemtulla A, Lyssiotis CA, Pennathur S, Reddy P. Nat Immunol. 2021 Nov;22(11):1440-1451. doi: 10.1038/s41590-021-01048-3. Epub 2021 Oct 22. PMID: 34686860.
Abstract

ATDC binds to KEAP1 to drive NRF2-mediated tumorigenesis and chemoresistance in pancreatic cancer
Purohit V, Wang L, Yang H, Li J, Ney G.M,Gumkowski E.R, Vaidya A.J, Wang A, Bhardwaj A, Zhao E, Dolgalev I, Zamperone A, Abel EV, Pasca Di Magliano M, Crawford HC, Diolaiti D, Papagiannakopoulos TY, Lyssiotis CA, Simeone DM. Genes Dev. 2021 Jan 14. doi: 10.1101/gad.344184.120. Epub ahead of print.
Abstract

Hyaluronic acid fuels pancreatic cancer cell growth
Kim PK, Halbrook CJ, Kerk SA, Radyk M, Wisner S, Kremer DM, Sajjakulnukit P, Andren A, Hou SW, Trivedi A, Thurston G, Anand A, Yan L, Salamanca-Cardona L, Welling SD, Zhang L, Pratt MR, Keshari KR, Ying H, Lyssiotis CA. Elife. 2021 Dec 24;10:e62645. doi: 10.7554/eLife.62645. PMID: 34951587; PMCID: PMC8730721.
Abstract

Medium-Chain Acyl-CoA Dehydrogenase Protects Mitochondria from Lipid Peroxidation in Glioblastoma
Puca F, Yu F, Bartolacci C, Pettazzoni P, Carugo A, Huang-Hobbs E, Liu J, Zanca C, Carbone F, Del Poggetto E, Gumin J, Dasgupta P, Seth S, Srinivasan S, Lang FF, Sulman EP, Lorenzi PL, Tan L, Shan M, Tolstyka ZP, Kachman M, Zhang L, Gao S, Deem AK, Genovese G, Scaglioni PP, Lyssiotis CA, Viale A, Draetta GF. Cancer Discov. 2021 Nov;11(11):2904-2923. doi: 10.1158/2159-8290.CD-20-1437. Epub 2021 May 26. PMID: 34039636; PMCID: PMC8711129.
Abstract

Apolipoprotein E Promotes Immune Suppression in Pancreatic Cancer through NF-κB-Mediated Production of CXCL1
Kemp SB, Carpenter ES, Steele NG, Donahue KL, Nwosu ZC, Pacheco A, Velez-Delgado A, Menjivar RE, Lima F, The S, Espinoza CE, Brown K, Long D, Lyssiotis CA, Rao A, Zhang Y, Pasca di Magliano M, Crawford HC.  Cancer Res. 2021 Aug 15;81(16):4305-4318. doi: 10.1158/0008-5472.CAN-20-3929. Epub 2021 May 28. PMID: 34049975; PMCID: PMC8445065.
Abstract

EWS-FLI1 and Menin Converge to Regulate ATF4 Activity in Ewing Sarcoma
Jiménez JA, Apfelbaum AA, Hawkins AG, Svoboda LK, Kumar A, Ruiz RO, Garcia AX, Haarer E, Nwosu ZC, Bradin J, Purohit T, Chen D, Cierpicki T, Grembecka J, Lyssiotis CA, Lawlor ER. Mol Cancer Res. (2021) Jul;19(7):1182-1195.
doi: 10.1158/1541-7786.MCR-20-0679. Epub 2021 Mar 19. PMID: 33741715; PMCID: PMC8462528.
Abstract | PDF

Multimodal Mapping of the Tumor and Peripheral Blood Immune Landscape in Human Pancreatic Cancer. Steele NG, Carpenter ES, Kemp SB, Sirihorachai V, The S, Delrosario L, Lazarus J, Amir ED, Gunchick V, Espinoza C, Bell S, Harris L, Lima F, Irizarry-Negron V, Paglia D, Macchia J, Chu AKY, Schofield H, Wamsteker EJ, Kwon R, Schulman A, Prabhu A, Law R, Sondhi A, Yu J, Patel A, Donahue K, Nathan H, Cho C, Anderson MA, Sahai V, Lyssiotis CA, Zou W, Allen BL, Rao A, Crawford HC, Bednar F, Frankel TL, Pasca di Magliano M.  Nat Cancer. 2020 Nov;1(11):1097-1112. doi: 10.1038/s43018-020-00121-4. Epub 2020 Oct 26. PMID: 34296197; PMCID: PMC8294470.
Abstract

Cancer SLC43A2 alters T cell methionine metabolism and histone methylation.
Bian Y, Li W, Kremer DM, Sajjakulnukit P, Li S, Crespo J, Nwosu ZC, Zhang L, Czerwonka A, Pawłowska A, Xia H, Li J, Liao P, Yu J, Vatan L, Szeliga W, Wei S, Grove S, Liu JR, McLean K, Cieslik M, Chinnaiyan AM, Zgodziński W, Wallner G, Wertel I, Okła K, Kryczek I, Lyssiotis CA, Zou W. Nature. 2020 Sep;585(7824):277-282. doi: 10.1038/s41586-020-2682-1.
Abstract | PDF

Integrated Metabolic and Epigenomic Reprograming by H3K27M Mutations in Diffuse Intrinsic Pontine Gliomas. 
Chung C, Sweha SR, Pratt D, Tamrazi B, Panwalkar P, Banda A, Bayliss J, Hawes D, Yang F, Lee HJ, Shan M, Cieslik M, Qin T, Werner CK, Wahl DR, Lyssiotis CA, Bian Z, Shotwell JB, Yadav VN, Koschmann C, Chinnaiyan AM, Blüml S, Judkins AR, Venneti S. Nature. 2020 Sep;585(7824):277-282. doi: 10.1038/s41586-020-2682-1.
Abstract | PDF

Purine metabolism regulates DNA repair and therapy resistance in glioblastoma.
Zhou W, Yao Y, Scott AJ, Wilder-Romans K, Dresser JJ, Werner CK, Sun H, Pratt D, Sajjakulnukit P, Zhao SG, Davis M, Nelson BS, Halbrook CJ, Zhang L, Gatto F, Umemura Y, Walker AK, Kachman M, Sarkaria JN, Xiong J, Morgan MA, Rehemtualla A, Castro MG, Lowenstein P, Chandrasekaran S, Lawrence TS, Lyssiotis CA, Wahl DR. Nat Commun. 2020 Jul 30;11(1):3811. doi: 10.1038/s41467-020-17512-x.
Abstract | PDF

Histone Acetyltransferase MOF Regulates Fatty Acid Oxidation and Cellular Metabolism in Cell Fate Determination.
Khoa LTP, Tsan Y-C, Mao F, Kremer MD, Sajjakulnukit P, Tong X, Bhanu NV, Garcia BA, Yin L, Smith GD, Saunders TL, Bielas S, Lyssiotis CA, Dou Y. Cell Stem Cell, 2020;20:30272-1.
Abstract

Common biochemical properties of metabolic genes recurrently dysregulated in tumors.
Oruganty K, Campit SE, Mamde S, Lyssiotis CA, Chandrasekaran S. Cancer & Metabolism, 2020;8:5.
Abstract | PDF

Integration of high-content fluorescence imaging into the metabolic flux assay reveals insights into mitochondrial properties and functions.
Little AC, Kovalenko I, Goo LE, Hong HS, Kerk S, Yates JA, Purohit V, Lombard D, Merajver S, Lyssiotis CA. Communications Biology, 2020;3:271.
Abstract | PDF

Endoplasmic reticulum-associated degradation regulates mitochondrial dynamics in brown adipocytes.
Zhou Z, Torres M, Sha H, Halbrook CJ, Van den Bergh F, Reinert RB, Yamada T, Wang S, Luo Y, Hunter AH, Wang C, Sanderson TH, Liu M, Taylor A, Sesaki H, Lyssiotis CA, Wu J, Kersten S, Beard DA, Qi L. Science, 2020;368:54-60.
Abstract | PDF

Severe metabolic alterations in liver cancer lead to ERK pathway activation and drug resistance.
Nwosu Z, Piorońska W, Battello N, Zimmer A, Dewidar B, Han M, Pereira S, Blagojevic B, Holenya P, Lochead J, De La Torre C, Gretz N, Sajjakulnukit P, Zhang L, Marquardt J, Pasca Di Magliano M, Lyssiotis CA, Ebert M, Sleeman J, Wölfl S, Meyer C, Hofmann U, Dooley S. EBiomedicine, 2020;102699.
Abstract

Small molecule activation of metabolic enzyme pyruvate kinase muscle isozyme 2, PKM2, provides photoreceptor neuroprotection.
Wubben TJ, Pawar M, Weh E, Smith A, Sajjakulnukit P, Zhang L, Dai L, Hager H, Pai MP, Lyssiotis CA, Besirli CG. Scientific Reports, 2020;10:2990.
Abstract | PDF

Cysteine depletion induces pancreatic tumor ferroptosis in mice.
Badgley MA, Kremer DM, Maurer HC, DelGiorno KE, Lee H-J, Purohit V, Sagalovskiy I, Firl CEM, Sastra SA, Palermo CF, Kapillian J, Decker A, Ma A, Sajjakulnukit P, Zhang L, Tolstyka ZP, Cerf TH, Liu T, Gu W, Seeley ES, Stone E, Georgiou G, Luga A, Wahl GM, Stockwell BR, Lyssiotis CA, Olive KP. Science, 2020;368:85-89.
Abstract | PDF

Regulatory T cell depletion causes compensatory immune suppression and accelerated pancreatic carcinogenesis.
Zhang Y, Lazarus J, Steele N, Lee H-J, Yan W, Halbrook CJ, Menjivar R, Kemp SB, Sirihorocai V, Carpenter ES, Nevison AC, Vinta A, Crawford HC, Lyssiotis CA, Frankel TL, Bednar F, Pasca di Magliano M. Cancer Discovery, 2020;10:422-439.
Abstract | PDF

Tissue of Origin Dictates GOT1 Dependence and Confers Synthetic Lethality to Radiotherapy.
Nelson BS, Lin L, Kremer DM, Sousa C, Cotta-Ramusino C, Myers A, Ramos J, Gao T, Kovalenko I, Wilder-Romans K, Dresser J, Davis M, Nwosu Z, Mashadova O, Nicolay B, Tolstyka ZP, Halbrook CJ, Asara JM, Crawford HC, Cantley LC, Kimmelman AC, Wahl D, Lyssiotis CA. Cancer & Metabolism, 2020;8:1.
Abstract | PDF

A Ribose-Scavenging System Confers Colonization Fitness on the Human Gut Symbiont Bacteroides thetaiotaomicron in a Diet-Specific Manner.
Glowacki RWP, Pudlo NA, Tuncil Y, Luis AS, Sajjakulnukit P, Terekhov AI, Lyssiotis CA, Hamaker BR, Martens EC. Cell Host & Microbe, 2020;27:79-92
Abstract | PDF

A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth.
Yoshida T, Yamasaki S, Kaneko O, Taoka A, Tomimoto Y, Namatame I, Yahata T, Kuromitsu S, Cantley LC, Lyssiotis CA. BBRC, 2020;522:633-638
Abstract

Inhibiting the biosynthesis of UDP-GlcNAc lowers O-GlcNAcylated levels and sensitizes cancer to environmental stress.
Walter LA, Lin YH, Halbrook CJ, Chuh KN, He L, Pedowitz NJ, Batt AR, Brennan CK, Stiles BL, Lyssiotis CA, Pratt MR. Biochemistry, 2019 [ePub ahead of print]
Abstract | PDF

NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome.
Fang EF, Hou Y, Lautrup S, Jensen MB, Yang B, SenGupta T, Caponio D, Khezri R, Demarest TG, Aman Y, Figueroa D, Morevati M, Lee H-J, Kato H, Kassahun H, Lee J-H, Filippeli D, Okur MN, Mangerich A, Croteau DL, Maezawa Y, Lyssiotis CA, Tao J, Yokote K, Rusten TE, Mattson MP, Jasper H, Nilsen H, Bohr VA. Nature Communications, 2019;10:5284.
Abstract | PDF

Meta-analysis of targeted metabolomics data from heterogeneous biological samples provides insights into metabolite dynamics.
Lee H-J, Kremer DM, Sajjakulnukit P, Zhang L, Lyssiotis CA. Metabolomics, 2019;15:103.
Abstract | PDF

Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells
Libiad M, Bostelaar T, Vitvitsky V, Bak D, Lee H-J, Leprevost FdV, Sakamoto N, Nesvizhskii A, Chen G, Fearon E, Lyssiotis CA, Martens E, Werapana E, Banerjee R. JBC, 2019;294:12077-12090.
Abstract | PDF

Auditory metabolomics, an approach to identify acute molecular effects of noise trauma.
Ji L, Lee HJ, Wan G, Wang G, Zhang L, Sajjakulnukit P, Schacht J, †Lyssiotis CA [†co-senior authors], †Corfas G. Scientific Reports, 2019;9:9273.
Abstract | PDF

Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.
Li M, Chiang YL, Lyssiotis CA, Teater M, Hong JY, Shen H, Wang L, Hu J, Jing H, Chen Z, Jian N, Duy C, Mistry SJ, Cerchietti L, Cross JR, Cantley LC, Green MR, Lin H, Melnick AM. Cancer Cell, 2019;35:916-931.
Abstract

Abnormal oxidative metabolism in a quiet genomic backgorund underlies clear cell papillary renal cell carcinoma.
Xu J, Reznik E, Lee H-J, Gundem G, Jonsson P, Sarungbam J, Bialik A, Vega FS, Creighton C, Hoekstra JG,  Zhang L, Sajjakulnukit P, Kremer DM, Tolstyka ZP, Casuscelli J, Stirdivant S, Tang J, Schultz N, Jeng PS, Dong Y, Su W, Cheng E, Russo P, Coleman J, Papaemmanuil E, Chen YB, Reuter VE, Sander C, Kennedy S, Hsieh J, †Lyssiotis CA [†co-senior authors], †Tickoo SK, †Hakimi AA. eLife, 2019;8:e38986.
Abstract | Full Article

Enhanced oxidative phosphorylation by PLZF is essential for survival and function of NKT cells.
Kumar A, Pyaram K, Yarosz E, Hong H, Lyssiotis CA, Giri S, Chang C-H.PNAS, 2019;116:7439-7448.
Abstract | PDF

Macrophage Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer.
Halbrook CJ, Pontious C, Lee H-J, Kovalenko I, Zhang Y, Lapienyte L, Dreyer S, Kremer DL, Zhang L, Sajjakulnukit P, Zhang L, Nelson B, Hong H, Kemp S, Chang D, Biankin A, Crawford HC, Morton JP, Pasca di Magliano M, Lyssiotis CA. Cell Metabolism, 2019;29:1390-1399.
Abstract

ERK inhibition increases pancreatic cancer dependence on autophagy.
Bryant KL, Stalnecker CA, Zeitouni D, Klomp JE, Peng S, Tikunov AP, Gunda V, Pierobon M, Waters AM, George SD, Tomar G, Papke B, Hobbs GA, Yan L, Hayges TK, Diehl JN, Goode G, Chaika NV, Wang Y, Zhang G-F, Witkiewicz AK, Knudsen ES, Petricoin E, Singh PK, Macdonald JM, Tran NL, Lyssiotis CA, Ying H, Kimmelman AC, Cox AD, Der CJ Nature Medicine, 2019;25:628-640.
Abstract | Full Article

IDH1R132H acts as a tumor suppressor in glioma via epigenetic upregulation of the DNA damage response.
Núñez FJ, Mendez FM, Kadiyala P, Savelieff MG, Koschmann C, Calinescu A-A, Kamran N, Patel R, Carney S, Alghamri M, Edwards M, Ljungman M, Qin T, Sartor MA, Tagett R, Venneti S, Brosnan-Cashman J, Meeker A, Gorbunova V, Zhao L, Kremer DM, Zhang L, Lyssiotis CA, Hervey-Jumper S, Figueroa ME, Lowenstein PR, Castro MG. Science Translational Medicine, 2019;11:479.
Abstract | PDF

Protocol for 13C/15N Targeted Flux Analysis by LC-MS/MS for Ex Vivo and In Vivo Labeling of Central Carbon Metabolism and Related Pathways.
Yuan M, Kremer DM, Huang H, Ben Sahra I, Manning BD, †Lyssiotis CA [†co-senior authors], †Asara JM.  Nature Protocols, 2019;14:313-330.
Abstract | PDF

Oncogenic Kras supports pancreatic cancer through regulation of nucleotide synthesis.
Santana-Codinab N, Roeth AA, Zhang Y, Yang A, Asara JM, Wang X, Bronson RT, Lyssiotis CA, Ying H, Kimmelman AC. Nature Communications, 2018;9:4945.
Abstract | PDF

Tumor Crosstalk Networks Promote Growth and Support Immune Evasion in Pancreatic Cancer.
Halbrook CJ, Pasca di Magliano M, Lyssiotis CA. Am J Physiol Gastrointest Liver Physiol (2018) 315:G27-G35.
Abstract

Biochemical Characterization and Structure-based Mutational Analysis Provides Insight into Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors.
Holt MC, Assar Z, Beheshti Zavareh R, Lin L, Anglin J, Mashadova O, Haldar D, Mullarky E, Kremer DM, Cantley LC, Kimmelman AC, Stein AJ, Lairson LL, Lyssiotis CA. Biochemistry (2018) EPub Ahead of Print.
Abstract | PDF

Metabolic Interactions in the Tumor Microenvironment
Lyssiotis CA, Kimmelman AC. Trends in Cell Biology (2017) 27:863-875.
Abstract | PDF

Employing Metabolism to Improve the Diagnosis and Treatment of Pancreatic Cancer
Halbrook CJ, Lyssiotis CA. Cancer Cell (2017) 31:5-19.
Abstract

Pancreatic Stellate Cells Support Tumour Metabolism Through Autophagic Alanine Secretion.
Sousa CM, Biancur DE, Wang X, Halbrook CJ, Sherman MH, Zhang L, Kremer D, Hwang RF, Witkiewicz AK, Ying H, Asara JM, Evans RM, Cantley LC, *Lyssiotis CA [co-senior authors], *Kimmelman AC. Nature (2016) 25, 479-483.
Abstract

Targeting Glutamine Metabolism Sensitizes Pancreatic Cancer to PARP1-driven Metabolic Catastrophe Induced by ß-lapachone.
Chakrabarti G, Moore Z, Luo X, Ilcheva M, Ali A, Anderson GG, Padanad M, Scaglioni PP, Cantley LC, Kimmelman AC, DeBerardinis RJ, *Lyssiotis CA [co-senior authors], *Boothman DA. Cancer & Metabolism (2015) 3, 12.
Abstract

Oncogene Ablation-Resistant Pancreatic Cancer Cells Depend on Mitochondrial Function.
Viale A, Pettazzoni P, Lyssiotis CA, Ying H, Sanchez N, Marchesini M, Carugo A, Green T, Seth S, Giuliani V, Kost-Alimova M, Muller F, Colla S, Nezi L, Genovese G, Deem AK, Kapoor A, Carugo A, Yao W, Brunetto E, Kang Y, Yuan M, Asara JM, Wang YA, Heffernan TP, Kimmelman AC, Wang H, Fleming J, Cantley LC, DePinho R & Draetta G. Nature (2014) 30, 628–632.
Abstract | Times cited: 1244

Glutamine Supports Pancreatic Cancer Growth Through a KRAS-Regulated Metabolic Pathway.
*Son J, *Lyssiotis CA [*co-lead authors], Ying H, Wang X, Hua S, Ligorio M, Perera RM, Ferrone CR, Mullarky E, Shyh-Chang N, Kang Y, Fleming JB, Bardeesy N, Asara JM, Haigis MC, DePinho RA, Cantley LC & Kimmelman AC. Nature (2013) 496, 101–105.
Abstract | Times cited: 1997

Oncogenic Kras Maintains Pancreatic Tumors through Regulation of Anabolic Glucose Metabolism.
*Ying H, *Kimmelman AC, *Lyssiotis CA [*co-lead authors], Hua S, Chu GC, Fletcher-Sananikone E, Locasale JW, Son J, Zhang H, Coloff JL, Yan H, Wang W, Chen S, Viale A, Zheng H, Paik J, Lim C, Guimaraes AR, Martin ES, Chang J, Hezel AF, Perry SR, Hu J, Gan B, Xiao Y, Asara JM, Weissleder R, Wang YA, Chin L, Cantley LC & DePinho RA. Cell (2012) 149, 656–670.
Abstract | Times cited: 2076

Biochemical Characterization and Structure-based Mutational Analysis Provides Insight into Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors
Holt MC, Assar Z, Beheshti Zavareh R, Lin L, Anglin J, Mashadova O, Haldar D, Mullarky E, Kremer DM, Cantley LC, Kimmelman AC, Stein AJ, Lairson LL, Lyssiotis CA. Biochemistry (2018) EPub Ahead of Print.
Abstract | PDF

Metabolic Interactions in the Tumor Microenvironment
Lyssiotis CA, Kimmelman AC. Trends in Cell Biology (2017) 27:863-875.
Abstract

Employing Metabolism to Improve the Diagnosis and Treatment of Pancreatic Cancer
Halbrook CJ, Lyssiotis CA. Cancer Cell (2017) 31:5-19.
Abstract

Pancreatic Stellate Cells Support Tumour Metabolism Through Autophagic Alanine Secretion
Sousa CM, Biancur DE, Wang X, Halbrook CJ, Sherman MH, Zhang L, Kremer D, Hwang RF, Witkiewicz AK, Ying H, Asara JM, Evans RM, Cantley LC, *Lyssiotis CA [co-senior authors], *Kimmelman AC. Nature (2016) 25, 479-483.
Abstract

Targeting Glutamine Metabolism Sensitizes Pancreatic Cancer to PARP1-driven Metabolic Catastrophe Induced by ß-lapachone
Chakrabarti G, Moore Z, Luo X, Ilcheva M, Ali A, Anderson GG, Padanad M, Scaglioni PP, Cantley LC, Kimmelman AC, DeBerardinis RJ, *Lyssiotis CA [co-senior authors], *Boothman DA. Cancer & Metabolism (2015) 3, 12.
Abstract

Oncogene Ablation-Resistant Pancreatic Cancer Cells Depend on Mitochondrial Function
Viale A, Pettazzoni P, Lyssiotis CA, Ying H, Sanchez N, Marchesini M, Carugo A, Green T, Seth S, Giuliani V, Kost-Alimova M, Muller F, Colla S, Nezi L, Genovese G, Deem AK, Kapoor A, Carugo A, Yao W, Brunetto E, Kang Y, Yuan M, Asara JM, Wang YA, Heffernan TP, Kimmelman AC, Wang H, Fleming J, Cantley LC, DePinho R & Draetta G. Nature (2014) 30, 628–632.
Abstract | Times cited: 1244

Glutamine Supports Pancreatic Cancer Growth Through a KRAS-Regulated Metabolic Pathway
*Son J, *Lyssiotis CA [*co-lead authors], Ying H, Wang X, Hua S, Ligorio M, Perera RM, Ferrone CR, Mullarky E, Shyh-Chang N, Kang Y, Fleming JB, Bardeesy N, Asara JM, Haigis MC, DePinho RA, Cantley LC & Kimmelman AC. Nature (2013) 496, 101–105.
Abstract | Times cited: 1997

Oncogenic Kras Maintains Pancreatic Tumors through Regulation of Anabolic Glucose Metabolism
*Ying H, *Kimmelman AC, *Lyssiotis CA [*co-lead authors], Hua S, Chu GC, Fletcher-Sananikone E, Locasale JW, Son J, Zhang H, Coloff JL, Yan H, Wang W, Chen S, Viale A, Zheng H, Paik J, Lim C, Guimaraes AR, Martin ES, Chang J, Hezel AF, Perry SR, Hu J, Gan B, Xiao Y, Asara JM, Weissleder R, Wang YA, Chin L, Cantley LC & DePinho RA. Cell (2012) 149, 656–670.
Abstract | Times cited: 2076