Pricing for Structure, Composition & Histology Core
Services Offered and Prices
ex vivo nanoCT and microCT
For P30 members, 20% of the cost for ex vivo nanoCT and microCT scanning is covered by the core center.
The cost structure allows P30 members to choose the system based on scientific need rather than price.
All rates shown below are for UofM faculty. For academic users outside of UofM or Industry users, please contact contact Andrea Clark ([email protected] for nanoCT scans) or Michelle Lynch ([email protected] for microCT scans) for current rates. Prices shown are effective 8/1/2024.
Procedure: Acquisition by Core
Core Charges (non-members): $53.00/hour
P30 Member Cost: $42.40/hour
Procedure: Analysis by Core
Core Charges (non-members): $53.00/hour
P30 Member Cost: $42.40/hour
Procedure: Technician Time
Core Charges (non-members): $54.00/hour
P30 Member Cost: $43.20/hour
Procedure: Acquisition by Core
Core Charges (non-members): $61.00/hour
P30 Member Cost: $48.80/hour
Procedure: Analysis by Core
Core Charges (non-members): $61.00/hour
P30 Member Cost: $48.80/hour
Procedure: Expediated Acquisition by Core
Core Charges (non-members): $122.00/hour
P30 Member Cost: $122.00/hour
Procedure: Expediated Analysis by Core
Core Charges (non-members): $122.00/hour
P30 Member Cost: $122.00/hour
Raman Spectroscopy
The Composition Core can help you analyze specimens using Raman spectroscopy, which can detect the biochemical makeup of tissues and cells. It can give you semi-quantitative information on minerals (hydroxyapatite and brushite), proteins (collagen and myosin), lipids (phospholipids, cholesterol, unsaturated and trans fatty acids), sugars (glycosaminoglycans), and/or nucleic acids (RNA). This technique works well for both soft and hard tissues, including biomaterials. Contact Dr. Gurjit Mandair ([email protected]) for more information on analyzing your specimens, as well as this flyer for more information on Raman spectroscopy.
Procedure: Sample prep guidance
Core Charges (non-members): N/A
P30 Member Cost: free
Procedure: Sample sectioning
Core Charges (non-members): N/A
P30 Member Cost: see Histology section of Core
Procedure: Acquistion, Analysis
Core Charges (non-members): free
P30 Member Cost: free (priority scheduling)
Cryosectioning, Paraffin and Plastic Embedding & Sectioning Services
See the Histology Workflow and Standard Operating Procedures (SOPs) for more information on the Cryosectioning, Paraffin and Plastic embedding and sectioning services. The various workflows and SOPs describe the prep work, steps, and where each of the services listed below (items 3 & 4) would be involved in the process. Also available is a booklet containing all protocols for plastic thin-sectioning and histomorphometric analysis of the sections. Please note that plastic services have longer turnaround times due to extended processing and the slow polymerization of poly(methyl methacrylate).
Histology Requests
The Histology Core is using iLab to process histology requests. Requests for service may be submitted on the UMICH Orthopaedic Research Laboratories (ORL) Histology Core iLab page. Help or general information on using the iLab site can be found here.
NOTE: All rates shown below are for UofM faculty. For academic users outside of UofM or Industry users, please contact Carol Whitinger ([email protected]) or Emma Snyder-White ([email protected]) for current rates. Prices shown are effective 8/1/2024.
Labor Rates for Miscellaneous Services
- Plastic Services $57.40 / hour
- Paraffin Services $44.10 / hour
Full Service Histology
Services provided byMiMHC Core personnel
Process + Embed in Plastic – small (i.e. mouse bone)
Units or Notes: Per block
Core Charges (non-members): $53.50
Core Charges for P30 Members: $15.97
Process + Embed in Plastic - large (i.e. human bone)
Units or Notes: Per block
Core Charges (non-members): $109.10
Core Charges for P30 Members: $68.30
Cut thin plastic sections
Units or Notes: Per slide (usually 5μm)
Core Charges (non-members): $15.80
Core Charges for P30 Members: $5.94
Cut thicker plastic sections
Units or Notes: Per slide (usually 200μm)
Core Charges (non-members): $16.00
Core Charges for P30 Members: $7.79
Goldner’s Masson Trichrome stain
Units or Notes: Per slide
Core Charges (non-members): $5.10
Core Charges for P30 Members: $2.73
TRAP stain plastic sections
Units or Notes: Per run of 10 slides
Core Charges (non-members): $164.00
Core Charges for P30 Members: $39.51
Deplasticize/coverslip
Units or Notes: Per run of 10 slides
Core Charges (non-members): $31.60
Core Charges for P30 Members: $31.60
Miscellaneous tasks/protocol development
Units or Notes: Labor costs
Core Charges (non-members): $57.40 / hour
Core Charges for P30 Members: $57.40 / hour
Tissue cassetting
Units or Notes: Per block
Core Charges (non-members): $3.00
Core Charges for P30 Members: $3.00
Paraffin processing
Units or Notes: Per block
Core Charges (non-members): $4.10
Core Charges for P30 Members: $4.10
Paraffin embedding
Units or Notes: Per block
Core Charges (non-members): $2.50
Core Charges for P30 Members: $2.50
Paraffin sectioning*
Units or Notes: Per slide
Core Charges (non-members): $5.60
Core Charges for P30 Members: $5.60
Paraffin staining, basic
Units or Notes: Per slide
Core Charges (non-members): $9.10
Core Charges for P30 Members: $9.10
TRAP stain paraffin sections
Units or Notes: Per run of 10 slides
Core Charges (non-members): $109.40
Core Charges for P30 Members: $109.40
Decalcify tissues
Units or Notes: Per run of 20 samples
Core Charges (non-members): $114.70
Core Charges for P30 Members: $114.70
Miscellaneous tasks/protocol development
Units or Notes: Labor costs
Core Charges (non-members): $44.10 / hour
Core Charges for P30 Members: $44.10 / hour
Equipment rental
Units or Notes: Costs
Core Charges (non-members): $13.60 / hour
Core Charges for P30 Members: No charge
* Step-sectioning may incur a premium charge for the extra labor
Cryosectioning
Units or Notes: Per slide
Core Charges (non-members): $16.90
Core Charges for P30 Members: $16.90
Self Service Histology
Training provided by MiMHC Core personnel; only trained users can use sectioning equipment
Cut thicker plastic sections
Units or Notes: Per slide
Core Charges (non-members): $6.10
Core Charges for P30 Members: No charge
Paraffin embedding
Units or Notes: Per block
Core Charges (non-members): $1.10
Core Charges for P30 Members: $1.10
Use of microtome or cryostat
Units or Notes: Per hour
Core Charges (non-members): $13.60
Core Charges for P30 Members: No charge
Advanced Training
Training provided by MiMHC Core personnel and includes the proper use of equipment, safety training, review of work, and quality assurance.
An advanced histological training program has been developed to provide for the safe and proficient use of histological equipment. In addition to the training, supervision of first-time use is provided to ensure that all safety measures are being followed and the equipment is being used properly. Each training session comes with future technical support, slide Q & A, staining advice, etc. Our goal is to have trainees and staff not just know how to produce high quality histological outcomes, but to also improve their understanding of what they are doing on a scientific level. Trainees are welcome to ask our Histologists anything about the process, how to better prepare samples, and how to improve sample processing, etc. We also have a stock of control tissues that is available to accelerate the training.
Procedure: Up to 4 hours
Core Charges (non-members): $176.40 / person
Core Charges for P30 Members: depends on equipment
NOTE: All rates shown above are for UofM faculty. For academic users outside of UofM or Industry users, please contact Carol Whitinger ([email protected]) or Emma Snyder-White ([email protected]) for current rates. Prices shown are effective 8/1/2024.
Histological Staining Menu
Histological Staining Menu Photos
Image 1: Goldner's Masson Trichrome, mouse long bone, thin plastic, 200X
Image 2: Toluidine Blue, mouse tibia, paraffin, 100X
Image 3: Toluidine Blue - Basic Fuchsin, sheep spine with metal implant, thick plastic, 40X
Image 4: Silver Stain, mouse bone and tendon, paraffin, 40X
Image 5: Masson's Trichrome, mouse intestine, paraffin 100X
Image 6: One-Step Goldner's Masson Trichrome, mouse long bone, thin plastic, 400X
Image 7: TRAP, mouse long bone, thin plastic, 200X
Image 8: H&E, mouse kidney, paraffin, 100X
Image 9: Toluidine Blue, human iliac crest biopsy, thin plastic, 200X
Image 10: Masson's Trichrome, mouse femur, paraffin, 100X
Image 11: Toluidine Blue, implant, thick plastic, 100X
Image 12: H&E, pig skin, thick plastic, 200X
Image 13: Sanderson's RBS, mouse femur, thick plastic, 200X
Image 14: TRAP, mouse vertebrae, paraffin, 200X
Image 15: SafO/Fast Green, mouse femur, paraffin, 100X
Image 16: TRAP, bone, thick plastic, 200X
Image 17: Picrosirius Red (Brightfield), mouse connective tissue, paraffin, 200X
Image 18: Von Kossa, mouse femur, thin plastic, 200X
Image 19: RGB Trichrome, mouse longbone, paraffin, 200X
Image 20: Carstairs, human urethra, paraffin, 100X
Image 21: Oil Red O, human tendon cross section, frozen, 40X
Image 22: Masson Trichrome with Verhoeff Counterstain, mouse artery, paraffin, 40X
Image 23: Alcian Blue, mouse trachea, 200X
Image 24: Toluidine Blue-Basic Fuchsin, mouse femur, thin plastic, 200X
Image 25: Masson Trichrome, mouse femur, thin plastic, 400X
Available For: Plastic Thin Sections
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin
Highlights: Fibrin
Photo: 20
Available for: Plastic Thin Sections
Highlights: Osteoid, excellent cellular detail
Photo: 1
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 8, 12
Available for: Plastic Thin Sections, Paraffin, Frozen Sections
Highlights: Cartilage
Photo: 15
Available for: Plastic Thin Sections, Paraffin
Highlights: Collagen
Photos: 5, 10, 25
Available for: Plastic Thin Sections, Paraffin
Highlights: Elastic fibers, collagen
Photo: 22
Available for: Plastic Thin Sections
Highlights: Osteoid, for volume analysis only
Photo: 6
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: Osteoclasts
Photos: 7, 14, 16
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 2, 11
Available for: Plastic Thin Sections, Plastic Thick Sections
Highlights: Differentiates bone and soft tissue
Photos: 3, 24
Available for: Plastic Thin Sections
Highlights: Calcium
Photo: 18
Available For: Plastic Thick Sections
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin
Highlights: Fibrin
Photo: 20
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 8, 12
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: Osteoclasts
Photos: 7, 14, 16
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 2, 11
Available for: Plastic Thin Sections, Plastic Thick Sections
Highlights: Differentiates bone and soft tissue
Photos: 3, 24
Available For: Paraffin
Available for: Paraffin
Highlights: Collagen-type distinction
Photo: 23
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin
Highlights: Fibrin
Photo: 20
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 8, 12
Available for: Plastic Thin Sections, Paraffin, Frozen Sections
Highlights: Cartilage
Photo: 15
Available for: Plastic Thin Sections, Paraffin
Highlights: Collagen
Photos: 5, 10, 25
Available for: Plastic Thin Sections, Paraffin
Highlights: Elastic fibers, collagen
Photo: 22
Available for: Paraffin
Highlights: Collagen, bifringence; amyloid
Photo: 17
Available for: Paraffin
Highlights: General tissues trichrome
Photo: 19
Available for: Paraffin
Highlights: Lacunae and osteocytic dendrites
Photo: 4
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: Osteoclasts
Photos: 7, 14, 16
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 2, 11
Available For: Frozen Sections
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 8, 12
Available for: Plastic Thin Sections, Paraffin, Frozen Sections
Highlights: Cartilage
Photo: 15
Available for: Frozen Sections
Highlights: Lipid
Photo: 21
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: Osteoclasts
Photos: 7, 14, 16
Available for: Plastic Thin Sections, Plastic Thick Sections, Paraffin, Frozen Sections
Highlights: General tissue structures
Photos: 2, 11
Additional Equipment & Services Provided by the Core
Stratec XCT 2000 L peripheral quantitative computed tomography (pQCT) imaging system (training required)
Advice on the use of Dragonfly software for image analysis, the use of fully convolutional neural networks (FCNNs) for image segmentation, and the development and validation of new FCNNs
Advice on the use of contrast agents and vascular perfusions to acquire 3D micro/nanoCT images of soft tissues
Access to Faxitron imaging in the Orthopaedic Research Laboratories
