Immune Monitoring

The Immune Monitoring Shared Resource team provides immunologic and biological support for ongoing research projects and clinical trials at the University of Michigan Rogel Cancer Center and the biomedical community at the University of Michigan.

Our services include measuring multiple cytokines in a single assay, determining cytokine concentrations in various biological specimens and monitoring immune cell activation through investigative analytic procedures, including:

• Luminex cytokine assays
• Large and small scale ELISA assays
• ELISPOT assays

• Human immune profiling panel for 29-color analysis of cells in suspension:
  • CD45, CCR6, CD19, CD127, CD38, IgD, CD11c, CD16, CCR4, CD123, TCRgd, CXCR5, CD3, CD45RA, CD27, CD28, CD66b, CXCR3, CD161, CD45RO, CD24, CCR7, CD8, CD25, CD20, HLA-DR, CD4, CD14, CD56
• Mouse immune profiling panel for 16-color analysis of cells in suspension:
  • Ly-6G, CD11c, CD69, CD45, CD11b, CD19, CD25, CD3, TER-119, CD62L, CD8, TCRb, NK1.1, CD44, CD4, B220
• Custom CyTOF antibody conjugation
• Data acquisition of cells in suspension using Helios CyTOF
• Image acquisition of tissue sections using Hyperion CyTOF

To learn more and get started, please visit and register at the CyTOF Core website.

Blood and tissue samples, including both plasma or serum and peripheral blood mononuclear cells (PBMC), from University of Michigan Rogel Cancer Center members are accepted for processing and temporary storage. Our team performs the following services:

• Ficoll separation and storage of plasma and PBMC
• Isolation and storage of plasma or serum (no PBMC)
• Tumor processing to single cell suspension
• Processing and storage of apheresis product