Information for genotyping and methylation profiling.
Using the Illumina BeadArray based assay, the Advanced Genomics Core performs high throughput custom genotyping, whole genome genotyping, copy number variation (CNV), cytogenetic analysis, and linkage analysis assays. The Core Genotyping Services for the Illumina BeadArray platform consist of the following:
- Verify DNA concentration with a PicoGreen/Qubit assay,
- Normalize sample
- Perform Illumina Genotyping assay,
- Record SNP loci intensities with an iScan Scanner,
- Create a GenomeStudio Project for DNA analysis, and
- Generate reports (i.e. genotypes, sample call rates, SNP call rates, etc).
Platform | Illumina iScan |
Primary Contact | Susan Dagenais, Ph.D. UM Advanced Genomics Core e-mail: [email protected] Phone: 734-615-8743 |
Typical Output | Typical call rates are >99% for all standard Illumina GoldenGate, Infinium, and LCG Array products. |
Sample Requirements |
DNA Concentration Request – dependent upon how the stock DNA concentration was determined.
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Typical Turnaround | Typical turnaround time is 1-3 weeks. |
Critical Information |
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More Information | Illumina Standard and Custom Genotyping Arrays |
We request 10-12µl of DNA at a concentration of 50ng/µl based on Qubit or PicoGreen dsDNA Quantification and NOT NanoDrop. If you do not have access to Qubit or PicoGreen dsDNA quantification kits, you may submit 25-30µl of DNA at a concentration of 100ng/µl based on NanoDrop. In this case, the Core will determine the concentration with Qubit/PicoGreen and perform the normalization for an additional charge.
Unless the client requests verification of concentrations or the sample concentrations submitted is based on NanoDrop, we do not verify the concentrations of samples.
Illumina does not recommend using WGA DNA. The use of WGA DNA may lead to lower call rates and allelic bias.
Yes, but DNA must be 2kb or greater in size.
No. The client must provide their own controls (i.e. HapMap controls, cross plate duplicates, etc)
We perform assays based on first come first served, but the typical turnaround time is 1.5-3 weeks. However, the time frame for completion of your project may be longer as it is dependent upon the length of the queue and the number of samples in your project.
Yes, we assay samples with the Illumina Infinium MethylationEPIC assay.
No. Either the Epigenomics Core performs the bi-sulfite conversions ([email protected]) or the client submits bi-sulfite converted DNAs using the EZ DNA Methylation™ Kit from Zymo Research and following incubation conditions in Appendix 6 (Catalog Nos. D5001 & D5002): https://files.zymoresearch.com/protocols/_d5001_d5002_ez_dna_methylationga_o_kit.pdf).
Yes. However, we request that the client perform the Illumina FFPE QC assay (Link:https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/infinium_assays/infinium_hd_ffpe_qc_assay/infinium_ffpe_dna_qc_assay_insert_15020981_c.pdf) before we order kits for the FFPE restoration and Illumina Genotyping or MethylationEPIC kits.
A minimum of 100ng in a maximum volume of 8µl (i.e. 12.5ng/µl) is required, but if available, you may use up to 250ng of DNA.
Core Processing fees include Tech time (labor) fees, consumables, and iScan time fees.
University of Michigan
2800 Plymouth Rd.
Ann Arbor, MI 48109-2800