The Thermo Scientific™ Orbitrap Fusion™ Tribrid™ high-resolution mass spectrometer combines the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture that delivers unprecedented depth of analysis. It enables life scientists analyzing even the most challenging low-abundance, high-complexity, or difficult samples to identify more compounds more quickly, quantify more accurately, and elucidate structures more thoroughly. The instrument can perform parallel CID, HCD, ETD, EThcD and ETciD fragmentation analysis to provide the most in-depth information about the complex samples. With its ability to perform multinotch-MS3 (SPS-MS3), Orbitrap Fusion provides more accurate results when using Tandem Mass Tag (TMT) approach for relative quantitation.

In its current setup, Orbitrap Fusion is used for standard qualitative and SPS-MS3 based relative quantitation using TMT.

Acquired with funding from the Protein Folding Disorder Initiative, a part of Michigan Medicine's strategic research initiative.

This analytical HPLC is used exclusively for off-line fractionation of the complex peptide mixture. Each of these fractions is further separated by a column in-line with the mass spectrometer providing a robust two-dimensional (2D) separation of peptides. This often provides more in-depth coverage of the proteome.