scientist using mass spectrometry technology

State-of-the-art technology

Orbitrap Fusion Tribrid with ETD

The Thermo Scientific™ Orbitrap Fusion™ Tribrid™ high-resolution mass spectrometer combines the best of quadrupole, Orbitrap, and ion trap mass analysis in a revolutionary Tribrid architecture that delivers unprecedented depth of analysis. It enables life scientists analyzing even the most challenging low-abundance, high-complexity, or difficult samples to identify more compounds more quickly, quantify more accurately, and elucidate structures more thoroughly. The instrument can perform parallel CID, HCD, ETD, EThcD and ETciD fragmentation analysis to provide the most in-depth information about the complex samples. With its ability to perform multinotch-MS3 (SPS-MS3), Orbitrap Fusion provides more accurate results when using Tandem Mass Tag (TMT) approach for relative quantitation.

In its current setup, Orbitrap Fusion is used for standard qualitative and SPS-MS3 based relative quantitation using TMT.

Acquired with funding from the Protein Folding Disorder Initiative, a part of Michigan Medicine's strategic research initiative.

Orbitrap Fusion Tribid used by the Proteomics Resource Facility
Q Exactive HF

The Thermo Scientific™ Q Exactive HF is a hybrid quadrupole-Orbitrap mass spectrometer featuring an ultra-high-field Orbitrap analyzer. While it is capable of only HCD fragmentation, QE HF is faster and provides better resolution for every scan. Apart from its use in performing standard data-dependent acquisition (DDA), QE HF can also be used for data-independent acquisition (DIA) and parallel reaction monitoring (PRM; targeted proteomics)

In its current setup QE HF is used for standard qualitative, DIA, PRM proteomic approaches.

Note: Both mass spectrometers are equipped with dedicated RSLC-nano (Dionex) UHPLCs and nanospray sources.

Q Exactive HF used by the Proteomics Resource Facility
1260 Infinity II ANALYTICAL HPLC (Agilent)

This analytical HPLC is used exclusively for off-line fractionation of the complex peptide mixture. Each of these fractions is further separated by a column in-line with the mass spectrometer providing a robust two-dimensional (2D) separation of peptides. This often provides more in-depth coverage of the proteome.

1260 Infinity II Analytical HPLC used by the Proteomics Resource Facility
Contact Us
Room 3554/3556
Medical Science Research Building (MSRB) II
1150 West Medical Center Drive
Ann Arbor, MI 48109
Phone:: 734-615-5722
About Us
The Proteomics Resource Facility is one of the Biomedical Research Core Facilities, and a part of the Medical School Office of Research, where our mission is to foster an environment of innovation and efficiency that serves the Michigan Medicine research community and supports biomedical science from insight to impact.