Stocking all GE Dharmacon pGIPZ V2L clones and a selection of lentiviral miRNA/shRNA clones in the pGIPZ V3L, pTRIPZ, and pLKO backbones.
| Service | Yield/Amount | Internal Price | External Price |
| shRNA Library Clone (in stock) | The Vector Core currently stocks all pGipZ V2L and a few other lentiviral miRNA/shRNA clones, including some pGipZ V3L, pTRIPZ and TRC pLKO.1 clones. The customer will receive a 60 mm streaked plate of each clone. | $35.00 | N/A |
| TRC pLKO.1 nonstock shRNA (Millipore Sigma) | Non-stock TRC pLKO.1 (Millipore Sigma) clones can be ordered through the Vector Core. Researchers will receive ~100 uL glycerol stock of the shRNA clone ordered. The clone will also be put into the Vector Core pLKO.1 repository. | $55.00 | N/A |
| Lentiviral shRNA tester samples (5 × 3 ml of supernatant) |
Tester samples are a “quick and dirty” product tailored toward screening several shRNA products. Each 3 ml tester prep requires 2.5 μg of purified proviral plasmid DNA*. The cost is for a set of five. However, if you do not have 5 constructs, multiple 3 ml preps of the same construct can be performed (2 of one construct and 3 of another, etc.). | $309.00 | $463.50 |
| Concentrated Lentiviral/Retroviral Production (small scale, VSVG) |
100 mls of supernatant concentrated to 10 mls of 10X (or as requested) in DMEM (or as requested); 80 μg of proviral plasmid required.* | $678.00 | $1,017.00 |
| Plasmid Midi Prep (100 μg) | Custom purified DNA prep. | $91.00 | $136.00 |
*Please submit the exact amount of DNA requested in separate microfuge tubes for each shRNA clone.
In-Stock Clones:
We have the full V2L version of the human and mouse pGIPZ shRNA libraries (clone ID starting with V2L, see below to identify these clones). In addition, we have a selected list of lentiviral shRNA clones from the pGIPZ (V3L), pTRIPZ and TRC pLKO libraries. This product comes as a bacterial streaked plate.
pGIPZ and pTRIPZ:
- Go to Horizon Discovery.
- Search for your gene of interest and click shRNA
- Select the pGIPZ or pTRIPZ library, click on “individual” and then click on “glycerol” to see a list of clones available in the library.
- Use the clone ID# to search the Vector Core list for available in-stock clones.
TRC pLKO.1:
- Go to Millipore Sigma.
- Search for your gene of interest, scroll until you find the gene information box which includes links for products and click on “shRNA”.
- Click pricing for “Bacterial Glycerol Stock” for either the human or mouse for the list of available clones in the Sigma library. Use the TRCN# to search the Vector Core list for available in-stock clones.
If we do not have the pLKO.1 clone in stock we can order it for you as a nonstock shRNA for $56.00 (includes free shipping).
shRNA Clones in Stock:
(search by clone ID or TRCN#)
Control plasmids available:
| Plasmid | Gene Target | Company | Catalog Number |
| pGIPZ Empty | N/A | Horizon Discovery | RHS4349 |
| pGIPZ Scrambled Non-Target | Scrambled Sequence | Horizon Discovery | RHS4346 |
| pGIPZ GAPDH Positive Control | GAPDH | Horizon Discovery | RHS4371 |
| pGIPZ EG5 positive control | EG5 | Horizon Discovery | RHS4480 |
| pTRIPZ Empty | N/A | Horizon Discovery | RHS4750 |
| pTRIPZ non-silencing control | Scrambled sequence | Horizon Discovery | RHS4743 |
| pLKO.1 Empty | N/A | Millipore Sigma | SHC001 |
| pLKO.1 Scrambled Non-Target All Species | Scrambled Sequence | Millipore Sigma | SHC016 |
| pLKO.1 Scrambled Non-Mammalian Target (May inhibit GFP Expression) | Scrambled Sequence | Millipore Sigma | SHC002 |
| pLKO.1 CMV turboGFP | Expresses GFP | Millipore Sigma | SHC003 |
| pLKO.1 Luciferase shRNA | targeting luciferase from Photinus pyralis (GenBank Accession No. M15077) | Millipore Sigma | SHC007 |
| pLKO.5 Scrambled Non-Target All Species | Scrambled Sequence | Millipore Sigma | SHC216 |
| pLKO.5 Scrambled Non-Mammalian Target (May inhibit GFP expression) | Scrambled Sequence | Millipore Sigma | SHC202 |
Antibiotic Resistance and Selection: pGipZ and pTripZ: Ampicillin (carbenicillin, 100 μg/ml) and Zeocin (25 μg/ml) for bacterial selection. Puromycin for mammalian selection. pLKO.1: Ampicillin (carbenicillin, 100 μg/ml) for bacterial selection. Puromycin for mammalian selection.
1150 West Medical Center Drive
Ann Arbor, MI 48109
