Advanced Model Validation
We combine cutting-edge PacBio long-read sequencing with custom, automated bioinformatics tools developed specifically for validating genome engineering experiments.
Accelerate Your Research with Powerful, Customized Validation
Researchers, core facilities, and biotech companies need confidence in the accuracy of their animal models and genetic edits. At TAMC, we combine cutting-edge PacBio long-read sequencing with custom, automated bioinformatics tools developed specifically for validating genome engineering experiments. Our robust pipelines deliver precise, detailed, and easy-to-read reports—taking the guesswork out of validation. Whether you’re working with transgenic models, CRISPR edits, or exploring epigenetic states, TAMC’s end-to-end approach empowers you with clear, actionable results.
Our Featured Services
TransgeneFinder: Unravel Random Transgene Integration
Random integration can hide critical genomic changes. TransgeneFinder leverages PacBio sequencing and our proprietary bioinformatics engine to:
- Pinpoint transgene insertion sites—even in challenging regions
- Identify local genomic rearrangements and estimate copy number
- Assess transgene methylation status
- Design custom PCR assays for genotyping
- Generate a comprehensive, easy-to-read report for distribution to clients
Submission Requirements:
- Biological specimen: Cells, tissues, or blood (fresh or frozen at -80°C)
- Species and background strain (e.g., mouse C57BL/6, rat SD)
- Estimated sequence of DNA used for transgene (plasmid or construct sequence)
CRISPR-seq: High-depth coverage, amplification free Validation for Every Edit
Validate even the most complex genome edits using CRISPR/Cas9 for targeted sequencing from genomic DNA.
- PacBio’s PureTarget prep and custom gRNA design for targeted, PCR-free long-read sequencing
- Automated analysis pipelines to validate edits, characterize complex alleles, and reveal epigenetic markers
- Highly multiplexed, cost-effective workflows
- Receive a comprehensive set of data outputs that enable fast, informed decision-making
Submission Requirements:
- Biological specimen: Cells, tissues, or blood (fresh or frozen at -80°C)
- Reference sequence for genome-edited allele (target region or edited sequence)
- Species and background strain (e.g., mouse C57BL/6, rat SD)
- Genomic region of interest for sequencing (specific locus, coordinates, or gene name)
Custom Consultations and Support
Our team supports you every step of the way:
- Free project design sessions
- Guidance on sample preparation, sequencing, and analysis interpretation
- Custom bioinformatics consultation for unique projects
Who Should Use These Services?
- Core facilities: Confirm insertion sites and copy numbers for models created in-house or by collaborators—with automated analysis and reporting
- Academic researchers: Validate CRISPR-based edits and complex alleles; get clear, actionable results
- Companies: Meet regulatory/scientific standards with robust reports
- Epigenetics & genome scientists: Explore targeted methylation and chromatin architecture—with visual summaries you can use immediately
Why Choose TAMC?
- Expertise: Decades of model generation and validation experience
- Technology: State-of-the-art sequencing, bespoke bioinformatics pipelines
- Automation: Streamlined tools and reporting tailored to your workflow
- Customization: Every project receives a personalized strategy and attention
Get Started
Ready for advanced validation and automated results you can trust?
Contact us for consultations about any of these services: [email protected].
Questions?
Contact Us
1150 West Medical Center Drive
Ann Arbor, MI 48109
